Shape and Specificity in Mammalian 15-Lipoxygenase Active Site
نویسندگان
چکیده
منابع مشابه
Molecular dioxygen enters the active site of 12/15-lipoxygenase via dynamic oxygen access channels.
Cells contain numerous enzymes that use molecular oxygen for their reactions. Often, their active sites are buried deeply inside the protein, which raises the question whether there are specific access channels guiding oxygen to the site of catalysis. Choosing 12/15-lipoxygenase as a typical example for such oxygen-dependent enzymes, we determined the oxygen distribution within the protein and ...
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Hugo: ALOX15 Other names: 15-LOX; EC 1.13.11.33; arachidonate omega 6-lipoxygenase; LOG15 Location: 17p13.3 Local order: Genes flanking ALOX15 in centromere to telomere direction on 17p13 are: MYBBP1A 17p13.3 MYB binding protein (P160) 1a, GGT6 17p13.2 homolog of gammaglutamyltransferase 6, LOC124974 17p13.2, thioredoxin 1 pseudogene 4, SMTNL2 17p13.2 smoothelin-like 2, ALOX15 17p13.2 arachidon...
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In the course of the lipoxygenase-catalyzed transformation of linoleic acid to 135-hydroperoxy-9Z,ll£'-octadecadienoic acid, iron ions are liberated. This iron release has been de termined using a spectrophotometric assay based on the complexation of ferrous iron by 3-(2-pyridyl)-5,6-bis-(4-phenylsulfonic acid)-l,2,4-triazine disodium salt (ferrozine). Further comparative measurements demonstr...
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The purified 15-lipoxygenase from rabbit reticulocytes is capable of oxidizing NADH in the presence of linoleic acid and oxygen. This co-oxidation proceeds at a rate that amounts to approx. 7% of linoleic acid oxygenation rates. Although NADH inhibits the lipoxygenase reaction with linoleic acid as substrate (46% inhibition at 0.2 mM NADH), the reaction specificity of the enzyme was not altered...
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Lipoxygenases (LOX) comprise a family of non-heme-iron-containing proteins that cata-lyze the dioxygenation of polyunsaturated fatty acids to form bioactive lipids. Metabolism of arachidonic acid by LOXs leads to the formation of regioisomeric cis/trans-conjugated hydroxyeicosatetraenoic acids (HeTes), leukot-rienes, lipoxins, and hepoxilins. Dependent on the predominant position of the incorpo...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1999
ISSN: 0021-9258
DOI: 10.1074/jbc.274.52.37345